This paper is published in Volume 2, Issue 4, 2017
Area
Pharmaceutical Analysis
Author
S. Nagajyothi
Co-authors
Y. Swetha, J. Neeharika, P. V Suresh, N. Ramarao
Org/Univ
Chalapathi Institute of Pharmaceutical Sciences, Andhra Pradesh, India
Pub. Date
17 April, 2017
Paper ID
V2I4-1168
Publisher
Keywords
Liquid Chromatography, Gas Chromatography, Infrared Spectroscopy, Mass Spectroscopy, NMR, Capillary Zone Electrophoresis.

Citationsacebook

IEEE
S. Nagajyothi, Y. Swetha, J. Neeharika, P. V Suresh, N. Ramarao. Hyphenated Techniques- A Comprehensive Review, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARnD.com.

APA
S. Nagajyothi, Y. Swetha, J. Neeharika, P. V Suresh, N. Ramarao (2017). Hyphenated Techniques- A Comprehensive Review. International Journal of Advance Research, Ideas and Innovations in Technology, 2(4) www.IJARnD.com.

MLA
S. Nagajyothi, Y. Swetha, J. Neeharika, P. V Suresh, N. Ramarao. "Hyphenated Techniques- A Comprehensive Review." International Journal of Advance Research, Ideas and Innovations in Technology 2.4 (2017). www.IJARnD.com.

Abstract

The hyphenated technique is the combination or the coupling of the different analytical techniques. Mainly chromatographic techniques are combined with spectroscopic techniques. Then the separated components of the mixture from chromatographic technique will enter into the spectroscopic technique through an interphase. In GC-MS the separated components from gas chromatography enter to MS which is followed by ionization, mass analysis, and detection of mass-to-charge ratios of ions generated from each analyze by the mass spectrometer. Jet/orifice separator, effusion separator, and membrane separator can be used to connect GC with MS. In LC- NMR coupling the analytical flow cell was initially constructed for continuous-flow to NMR. However, the need for full structural assessment of novel natural products has led to the application in the stopped-flow mode in LC-MS. Use of LC-MS-MS is increasing speedily day by day. Hyphenated techniques such as HPLC coupled to UV and mass spectrometry (LC-UV-MS) have been extremely useful in combination with biological screening for a rapid survey of natural products. Nowadays, various types of LC-MS systems incorporating different types of interfaces are available commercially. The term hyphenated techniques refer to separation, identification, and the hyphenated techniques shows better analysis of the samples are components specificity, accuracy, precision.
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