Biosurfactants are a structurally diverse group of surface-active substances synthesized by microorganisms. All biosurfactants are amphiphilic, they consist of two parts—a polar (hydrophilic) moiety and non polar (hydrophobic) group. These biosurfactants have tremendous applications. This review focuses on the varying microorganisms which has the potential ability to synthesize surface active substance, and the microorganisms which has the pivotal role to play in bioremediation.