Choosing a major subject at the undergraduate level can be an intricate choice and one that has the potential to determine an individual's professional future. The purpose of this study is to examine the impact of influencing factors that determine the business students’ choice of specialization or major subject selection. The study also examines the possible career options that are the desired outcome of choice of specialization. It will provide information for the higher education institutions to increase the number of students in their institutions. The study was conducted by collecting data from 170 respondents from four universities in the Sylhet region. Respondents were more concerned about careers in HRM, Marketing, Accounting, finance, and MIS. The study grasped that some factors like job prospects, the guidance of the teacher or instructor and parents’ opinion which has a great impact on choosing a specialization. We also found from this study that advisory support and family income have the least impact on choosing a particular specialization.